484-001 Luciferase Assay Kit is based on that the firefly luciferase luc gene is one of the most commonly used reporter genes and allows the monitoring of promoter activity in the control of gene expression. It has a number of advantages and is an interesting option to CAT assays and non-luminescent reporter gene assays:nn1) Firefly luciferase is not present in normal cells.nn2) The assay is simple and easy to perform.nn3) You can use both simple, manual, luminometers as well as fully automatic microplate luminometers.nn4) It is suitable for HTS.nnThe kit contains reagents for 800 assays. If you want to perform the assay as an HTS, the 484-102 LAS HTS Kit is suitable if you have a luminometer without dispensers and 484-103 LAS HTS is suitable if you have a luminometer with dispensers.nThe firefly luciferase luc gene is the most commonly used reporter gene for studying cellular events coupled to gene expression. It has a number of distinct advantages such as: no background, easy analytical procedure, extreme sensitivity (10-20 mole luciferase can be detected) and reliability of results. The reagents contain special activators to counteract any inactivation of the luciferase enzyme during cell culture or sample storage. All three Luciferase Assay Kits contain the same reagents. The High Throughput Screening kits (484-102 and 484-103) come in larger vials for easier handling.
产品描述
Luciferase Assay Kit is based on that the firefly luciferase luc gene is one of the most commonly used reporter genes and allows the monitoring of promoter activity in the control of gene expression.nnDetection limit:nnWith most luminometers 10 -19 moles of luciferase can be detected.
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BioThema成立于1987 年，總部位于瑞典斯德哥爾摩南郊，只做一件事：把萤火蟲荧光素酶反應做到極致。38 年來，公司依舊保持“小而專”的規模，卻憑這一件事成爲全球 ATP 與生物冷光分析的隱形冠軍。nBioThema致力于萤火蟲荧光素酶反應的分析應用。這種多功能反應已廣泛用于各種檢測，包括細胞數量定量、細胞增殖、細胞毒性、參與ATP形成或降解反應的所有酶和代謝物分析、報告基因檢測、免疫檢測、快速細胞計數和環境衛生監測。同時BioThema也是全球很多品牌的ATP和荧光素的OEM原料供應商；
