中文名稱

ATP 生物質試劑盒 HS

英文名字

ATP Biomass Kit HS

供應商

BioThema

产品貨號

BTA-266-311

产品報價

￥詢價/375tests

产品說明書

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購買方式

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产品新聞

背景資料

This kit is intended for determination of total ATP (adenosine triphosphate) in samples containing living cells. If the intention is to determine intracellular ATP one should consider using 266-111 Intracellular ATP Kit HS or 266-112 Microbial ATP Kit HS. These kits include an ATP Eliminating Reagent for degradation of extracellular ATP and the latter kit also a Cell Lysing Reagent for lysis of most animal cells. All living cells contain ATP where it plays the role of energy currency between different cellular processes.nnWhen cells die of natural causes, ATP is normally degraded. The intracellular concentration of ATP is carefully regulated to similar levels in all types of cells. ATP is therefore a good estimate of the total intracellular volume. Most bacterial cells contain approx. 2*10-18 mol ATP per cell, while most eukaryotic cells, as a result of their larger size, contain 10 -15 mol ATP or more. ATP Biomass Kit HS, Intracellular ATP Kit HS and Microbial ATP Kit HS contain ATP Reagent HS (High Sensitivity) with a high luciferase activity. The luciferase activity in ATP Reagent HS is actually so high that if contaminated during reconstitution it will degrade the ATP background by one order of magnitude every 38 min. After mixing sample and ATP Reagent HS the light intensity decays by approx. 6% per min.nnThis is slow enough to allow manual mixing and a tube luminometer without reagent dispenser can be used. The detection limit of the reagent is 10*10-18 mol ATP with a sensitive luminometer. This corresponds to 5 bacterial cells. We must here differentiate between bacterial cells and CFU, since one CFU may consist of several cells. If the detection limit is not adequate, it is possible to concentrate the sample by microfiltration, a procedure that will also remove most of the extracellular ATP.

产品描述

The ATP Biomass Kit is suitable for virtually all types of samples.nThe sensitivity of this kit allows determination of low ATP levels in a variety of circumstances:n1. Liquids (e. g. drinking water, process water, environmental samples, beverages and beer)n2. Pharmaceuticals and cosmeticsn3. Cell adhesion to surfaces (e. g. biocompatibility studies)n4. Quality assurance of laundryn5. Bacterial growth.n6. Antibiotic effects on bacteriannNumber of assays:nThe kit contains reagents for 150-375 assays (each vial of ATP Reagent HS is sufficient for 25 assays in a tube luminometer and 62.5 assays in a microplate luminometer).nnDetection limit:nThe detection limit of the reagent is 10×10 -18 mol ATP with a sensitive luminometer. This corresponds to 5 bacterial cells.

产品形式

保存建議

其他

BioThema成立于1987 年，總部位于瑞典斯德哥爾摩南郊，只做一件事：把萤火蟲荧光素酶反應做到極致。38 年來，公司依舊保持“小而專”的規模，卻憑這一件事成爲全球 ATP 與生物冷光分析的隱形冠軍。nBioThema致力于萤火蟲荧光素酶反應的分析應用。這種多功能反應已廣泛用于各種檢測，包括細胞數量定量、細胞增殖、細胞毒性、參與ATP形成或降解反應的所有酶和代謝物分析、報告基因檢測、免疫檢測、快速細胞計數和環境衛生監測。同時BioThema也是全球很多品牌的ATP和荧光素的OEM原料供應商；

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