Cell Viability Kit SL is intended for cell proliferation and cytotoxicity studies by determination of total ATP (adenosine triphosphate). Extractant B/S included in the kit releases ATP from most types of cells and inactivates ATP degrading enzymes. For maximum accuracy, the level of extracellular ATP should be low compared to the intracellular level.nnWith low numbers of cells, ATP degrading enzymes are not a problem and 155-050 Cellular ATP Kit HTS can be used for mammalian but not microbial cells. This kit allows a mix-and-measure assay with only one step (the Cell Viability Kit SL requires two steps – first extraction and then light measurement). If a higher sensitivity is required or with bacterial samples, the 266-311 ATP Biomass Kit HS, 266-111 Intracellular ATP Kit HS or 266-311 Microbial ATP Kit HS are recommended. Assays of ATP replace methods such as tritiated thymidine incorporation and tetrazolium salt reduction.nnThe Cell Viability Kit SL is useful for quantification of ATP in range of 10-11 – 10-7 mol/L. This dynamic range covers the commonly used seeding cell number and culturing media.nnAll living cells contain ATP where it plays the role of energy currency between different cellular processes. When cells die of natural causes, ATP is normally degraded. The intracellular concentration of ATP is carefully regulated to similar levels in all types of cells. ATP is therefore a good estimate of the total intracellular volume. Most bacterial cells contain approx. 2*10-18 mol ATP per cell, while most eukaryotic cells, as a result of their larger size, contain 10-15 mol ATP or more.nnThis kit can be used for determination of ATP levels in e.g. animal or bacterial cells in a variety of circumstances:nn1. Estimation of cell numbersnn2. Cell proliferation studiesnn3. Cytotoxicity studies
产品描述
Cell Viability Kit SL is intended for use with mammalian cell samples with high cell density or with cell samples with higher density of ATPases, e.g. nerve cells. The kit contains a stronger ATPase inhibitor than 155-050 Cellular ATP Kit HTS and produces stable light.nA mammalian cell contains approx. 1 femtomole ATP. 188-441 Cell Viablity Kit SL is useful for quantification of ATP in the range 10-11 – 10-7 mole/L.nNumber of tests:n320-1600 tests depending on whether assays are performed in cuvettes or microplates.nnDetection limit:nQuantitative detection of ATP in range of 10-11 – 10-7 mol/L.
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BioThema成立于1987 年，總部位于瑞典斯德哥爾摩南郊，只做一件事：把萤火蟲荧光素酶反應做到極致。38 年來，公司依舊保持“小而專”的規模，卻憑這一件事成爲全球 ATP 與生物冷光分析的隱形冠軍。nBioThema致力于萤火蟲荧光素酶反應的分析應用。這種多功能反應已廣泛用于各種檢測，包括細胞數量定量、細胞增殖、細胞毒性、參與ATP形成或降解反應的所有酶和代謝物分析、報告基因檢測、免疫檢測、快速細胞計數和環境衛生監測。同時BioThema也是全球很多品牌的ATP和荧光素的OEM原料供應商；
