Cellular ATP Kit HTS is intended for enumeration of viable cells by quantification of cellular ATP (adenosine triphosphate). The assay is intended for rapid analysis of cytotoxicity and proliferation in cell studies. The assay of cellular ATP replaces methods such as tritiated thymidine incorporation and tetrazolium salt reduction. If a stable light emission is not obtained, the ATPases are not completely inhibited. We then recommend the Cell Viability Kit SL containing a strongly ATPase inactivating agent.nnThe Cellular ATP Kit HTS is especially designed for HTS screening methodology; enabling assay in 96 well format with manual or fully automated handling. The assay format is a mix and measure addition of ATP Reagent containing cellular lysis agent and stabilizers for the firefly luciferase enzymatic reaction. The T1/2 of the light emission is 3 hours in medium containing 10% FCS (Fetal Calf Serum) and 12.5 hours in media supplemented with e.g. vitamin mixture F12, enabling screening of a large number of samples. The Cellular ATP Kit HTS is useful for quantification of ATP in range of 10-11 – 10-7 mol/L. This dynamic range covers the commonly used seeding cell number and culturing media.nnIn samples with a high density of ATPases, e.g. nerve cells, the ATPase inhibitor in the Cellular ATP Kit might not completly inhibit the ATPases. This will result in a slight decay of light. In such cases, a luminometer with dispensers could be used. Alternatively use 188-441 Cell Viability Kit SL which contains a stronger ATPase inhibitor.
产品描述
Cellular ATP Kit HTS is intended for use with mammalian cell samples. It is an easy-to-use kit where the lysing buffer, an ATPase inhibitor, and the ATP Reagent are combined in one reagent. The light emission is extremly stable, providing that all ATPases in the sample have been completly inhibited. That makes the 155-050 Cellular ATP Kit HTS suitable for high throughput screening.nNumber of assays:n1200 tests depending on whether assays are performed in microplates.nnDetection limit:nQuantitative detection of ATP in the range of 10–11 to 10–7 mol/L.
产品形式
保存建議
其他
BioThema成立于1987 年，總部位于瑞典斯德哥爾摩南郊，只做一件事：把萤火蟲荧光素酶反應做到極致。38 年來，公司依舊保持“小而專”的規模，卻憑這一件事成爲全球 ATP 與生物冷光分析的隱形冠軍。nBioThema致力于萤火蟲荧光素酶反應的分析應用。這種多功能反應已廣泛用于各種檢測，包括細胞數量定量、細胞增殖、細胞毒性、參與ATP形成或降解反應的所有酶和代謝物分析、報告基因檢測、免疫檢測、快速細胞計數和環境衛生監測。同時BioThema也是全球很多品牌的ATP和荧光素的OEM原料供應商；
