ATP Kit SL is used to assay both enzymes and metabolites. Any reaction possible to couple to ATP (adenosine triphosphate) formation or degradation, e.g. kinases and their substrates or mitochondrial ATP production, is possible to assay with the ATP Kit SL. ATP Kit SL contains ATP Reagent SL, a reagent emitting a stable light with a decay rate around 0.9 %/min. The stable light makes it possible to continuously monitor the ATP levels in a sample. At the end of the assay the measurement is calibrated with BioThemas internal ATP Standard. Both kinetic and end-point assays can be performed using the 144-041 ATP Kit SL.nnThe kit is intended for monitoring of ATP over the range of 10-12 to 10-6 mol/L. The low decay rate of the light emission is due to a low consumption of ATP in the firefly luciferase reaction and a luciferase activity that is unchanged during the time of the measurement. Under these circumstances measurement of the intensity of the light emission allows continuous monitoring of formation or degradation of ATP in enzymatic reactions or release of ATP from cells. Thus assays can be performed in much the same way as NAD(P)H is spectrophotometrically or fluorometrically monitored in assays based on dehydrogenase reactions. Furthermore the stable light makes assays even of a constant ATP level more convenient and reliable, particularly when using manual luminometers.nnIf a higher sensitivity is required BioThema has two other reagents allowing the detection of 10-17 mol and 10-18, respectively.nnIf no immediate reaction can be coupled to the metabolism of ATP, e.g. pyrosequencing or measurement of DNA polymerase, please contact us at info(at)biothema.com for recommendations of assay development.nnFor ATP determinations in eukaryotic cells we recommend Cell Viability Kit SL or Cellular ATP Kit HTS.nnFor ATP determinations in prokaryotic cells we recommend ATP Biomass Kit HS, Intracellular ATP Kit HS or Microbial ATP Kit HS, which all contain a more sensitive ATP reagent.
产品描述
ATP Kit SL is intended for assays of enzymes and metabolites by monitoring the formation or degradation of ATP (adenosine triphosphate) over the range of 10 –12 to 10 –6 mol/L.nNo. of determinations (cuvettes): 200nnNo. of determinations (microplate): 1000nnDetection limit: Quantitative detection of ATP in the range of 10–12 to 10-6 mol/L.nnDepending of the assay format this means 10–15 to 10–10 mol per assay (1 ml cuvette volume) for tube luminometers and 0.2*10–15 to 0.2*10–10 mol per assay (0.2 ml volume) for microplate luminometers.
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BioThema成立于1987 年，總部位于瑞典斯德哥爾摩南郊，只做一件事：把萤火蟲荧光素酶反應做到極致。38 年來，公司依舊保持“小而專”的規模，卻憑這一件事成爲全球 ATP 與生物冷光分析的隱形冠軍。nBioThema致力于萤火蟲荧光素酶反應的分析應用。這種多功能反應已廣泛用于各種檢測，包括細胞數量定量、細胞增殖、細胞毒性、參與ATP形成或降解反應的所有酶和代謝物分析、報告基因檢測、免疫檢測、快速細胞計數和環境衛生監測。同時BioThema也是全球很多品牌的ATP和荧光素的OEM原料供應商；
