Kinase RR Kit is based on a method of measuring the first order reaction rate constant of protein kinase reactions by monitoring the ATP degradation rate using bioluminescence. The assay starts by adding ATP to a reaction mixture containing all necessary assay components, including luciferase and luciferin. In HTS the light is measured at two points in time. In non-HTS the light can be measured repeatedly until a significan reduction of light is obtained.nnThe assay is set up so that:na) the kinase reaction follows first order kineticsnnb) the sample light emission, normalized against the blank (no kinase), is proportional to the remaining ATP concentration.nnThe first order rate constant of the kinase reaction is proportional to the kinase activity over several orders of magnitude, which makes it very easy to set up new assays. Furthermore variation in ATP and luciferase cancel out in calculations and do not affect assay results.
产品描述
Kinase RR Kit or Kinase Reaction rate Kit is developed for the easy and reliable identification of protein kinase inhibitor candidates from large compund libraries. The Kinase RR Kit uses direct bioluminescent measurement of ATP degradation rate to distingusih hits from non-hits. In contrast to other bioluminescent ATP based assays of kinase there is no interference from inhibitors acting on luciferase. Furthermore the standard curve is linerar rather than sigmodial. the Kinase RR Kit is flexible and can easily be adapted to various HTS strategies. Z’ values up to 0.96 have been reported. The assay does not require radioactive materials, antibodies or any form of conjugates.
产品形式
保存建議
4 °C
其他
BioThema成立于1987 年，總部位于瑞典斯德哥爾摩南郊，只做一件事：把萤火蟲荧光素酶反應做到極致。38 年來，公司依舊保持“小而專”的規模，卻憑這一件事成爲全球 ATP 與生物冷光分析的隱形冠軍。nBioThema致力于萤火蟲荧光素酶反應的分析應用。這種多功能反應已廣泛用于各種檢測，包括細胞數量定量、細胞增殖、細胞毒性、參與ATP形成或降解反應的所有酶和代謝物分析、報告基因檢測、免疫檢測、快速細胞計數和環境衛生監測。同時BioThema也是全球很多品牌的ATP和荧光素的OEM原料供應商；
